Device
Part:BBa_K1742012:Design
Designed by: Mónica Victoria Gutiérrez Salazar Group: iGEM15_Valencia_UPV (2015-09-07)
35S:Gal4BD-DronpaK:T35S - 35S:DronpaN-VP16:T35S
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3335
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 392
Illegal BglII site found at 1138
Illegal XhoI site found at 1342
Illegal XhoI site found at 1655 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1574
Design Notes
Do a PCR to obtain the Dronpa(145N)
Source
Synthesis